Virus RNA extraction kit (magnetic bead method)
【product name】
General Name: Virus RNA Extraction Kit (Magnetic Beads)
【expected usage】
This reagent box is suitable for extracting high purity viral nucleic acid [RNA) from a variety of samples such as human nasioles, sputum, bronchial lavette, alveolar lavage liquid.
The nucleic acid obtained can be used directly for reverse transcription, PCR, fluorescent quantitative PCR, RT-PCR, second-generation sequencing, biocontrol analysis and other downstream related experiments.
【working principle】
This kit uses a unique embedded ultra-metallic silicon base magnetic beads, in a unique buffer system, adsorbed nucleic acids through hydrogen bonds and electrostatic electrostatic, without adsorbing proteins and other impurities. The magnetic beads of the nucleic acid were washed to remove the remaining proteins and salts. When using low-salt buffers, the magnetic bead releases the nucleic acid to achieve a rapid separation of purified nucleic acid. The entire operation process is simple, fast and safe and efficient, and the extraction of the viral nucleic acid is high, the purity is high, and the quality is stable and reliable. It is suitable for automated extraction of high-throughput workstations.
Note:
1. Different batch reagent box components cannot be mixed.
2. Requireled reagents: no water ethanol.
3. Before using the initial use, please refer to the bottle tab to add the specified amount of absolute ethanol to the washing liquid and rinse liquid, and label.
4. The magnetic beads are needed before use, and the magnetic beads are fully dispersed.
5. If a pellet is produced in a fracture solution, it can be used at room temperature or 37 ° C for use.
6. When using this kit, please wear an experiment. [Primary composition of kits]
[Primary composition of kits]
| Component name | Specifications and quantities | instruction |
| Magnetic beads | 2ml * 1 tube | |
| Lysate | 60ml * 1 tube | Provide cracking and combined environment |
| Protease K | 1ml * 2 tube | Enzymatic solution |
| detergent | 38ml * 1 bottle | Remove the residual protein (50ml no water extraction before use) |
| Rinse liquid | 20ml * 1 bottle | Remove the residual salt ions (80 ml of no-water ethanol needed before use) |
| Eluate | 6ml * 1 bottle | Nucleophilicase solution |
[Sample requirements]
1. This extraction reagent is applicable to sample types: upper respiratory samples (including nasopharyngeal swabs, sputum); lower respiratory samples (including bronchi lavage, alveolar lavas).
2. Sample collection specific methods, please refer to the Microbiological Specimen Collection Manual or \"Clinical Nursing Practice Guide\".
3. After the sample collection, the extraction and test must be completed on the same day. Otherwise, save: 2 ~ 8 ° C Save, no more than 24 h;
4. Transportation: Use the foam tank to dry the ice seal for transportation.
[Storage conditions and validity period]
Store room temperature (15 ~ 25 ° C), valid for 6 months; longer preservation can be placed in 2 ~ 8 ° C, room temperature. The magnetic bead is recommended to store 2 ~ 8 ° C, pay attention to the magnetic beads not stored below 0 ° C.
Virus RNA extraction kit (magnetic bead method)
【product name】
General Name: Virus RNA Extraction Kit (Magnetic Beads)
【expected usage】
This reagent box is suitable for extracting high purity viral nucleic acid [RNA) from a variety of samples such as human nasioles, sputum, bronchial lavette, alveolar lavage liquid.
The nucleic acid obtained can be used directly for reverse transcription, PCR, fluorescent quantitative PCR, RT-PCR, second-generation sequencing, biocontrol analysis and other downstream related experiments.
【working principle】
This kit uses a unique embedded ultra-metallic silicon base magnetic beads, in a unique buffer system, adsorbed nucleic acids through hydrogen bonds and electrostatic electrostatic, without adsorbing proteins and other impurities. The magnetic beads of the nucleic acid were washed to remove the remaining proteins and salts. When using low-salt buffers, the magnetic bead releases the nucleic acid to achieve a rapid separation of purified nucleic acid. The entire operation process is simple, fast and safe and efficient, and the extraction of the viral nucleic acid is high, the purity is high, and the quality is stable and reliable. It is suitable for automated extraction of high-throughput workstations.
Note:
1. Different batch reagent box components cannot be mixed.
2. Requireled reagents: no water ethanol.
3. Before using the initial use, please refer to the bottle tab to add the specified amount of absolute ethanol to the washing liquid and rinse liquid, and label.
4. The magnetic beads are needed before use, and the magnetic beads are fully dispersed.
5. If a pellet is produced in a fracture solution, it can be used at room temperature or 37 ° C for use.
6. When using this kit, please wear an experiment. [Primary composition of kits]
[Primary composition of kits]
| Component name | Specifications and quantities | instruction |
| Magnetic beads | 2ml * 1 tube | |
| Lysate | 60ml * 1 tube | Provide cracking and combined environment |
| Protease K | 1ml * 2 tube | Enzymatic solution |
| detergent | 38ml * 1 bottle | Remove the residual protein (50ml no water extraction before use) |
| Rinse liquid | 20ml * 1 bottle | Remove the residual salt ions (80 ml of no-water ethanol needed before use) |
| Eluate | 6ml * 1 bottle | Nucleophilicase solution |
[Sample requirements]
1. This extraction reagent is applicable to sample types: upper respiratory samples (including nasopharyngeal swabs, sputum); lower respiratory samples (including bronchi lavage, alveolar lavas).
2. Sample collection specific methods, please refer to the Microbiological Specimen Collection Manual or \"Clinical Nursing Practice Guide\".
3. After the sample collection, the extraction and test must be completed on the same day. Otherwise, save: 2 ~ 8 ° C Save, no more than 24 h;
4. Transportation: Use the foam tank to dry the ice seal for transportation.
[Storage conditions and validity period]
Store room temperature (15 ~ 25 ° C), valid for 6 months; longer preservation can be placed in 2 ~ 8 ° C, room temperature. The magnetic bead is recommended to store 2 ~ 8 ° C, pay attention to the magnetic beads not stored below 0 ° C.
